Your technology with high sensitivity and maximum specificity. DNA or RNA is isolated from the specimen, amplified, and detected via a hybridization and alkaline phosphatase reaction on a membrane strip.
Following DNA isolation, nucleic acids are selectively replicated in an amplification reaction. In the next step, the amplicons are chemically denatured, since detection on the DNA•STRIP is done using single-stranded DNA. .
The DNA•STRIP is coated with highly specific probes which are complementary to selectively amplified nucleic acid sequences. The single-stranded amplicon binds specifically to the analog probes during hybridization, while non-specifically bound amplicons are removed in subsequent washing steps.
During the conjugate reaction, the specifically bound amplicon is marked with the enzyme alkaline phosphatase and is then made visible in a colorimetric detection reaction.
In this way, a specific banding pattern develops on the DNA•STRIP. Using a test-specific evaluation template, the test result can be read out quickly and clearly. Using this assay you can reliably determine the genotype or microbiological pathogen present as well as its resistances.
Your benefits with the DNA•STRIP technology
- Definite result: internal controls document the validity of the results and ensure accurate test execution.
- Low implementation costs: the technology does not require any costly equipment. Even small production runs can be efficiently processed at low costs.
- Can be automated for larger production runs: detection on the DNA•STRIP can be done manually as well as automatically. This allows optimal integration into laboratories routines of any size.
Efficient processing: all DNA•STRIP technology test systems can be combined with each other during processing. This permits joint execution of several human-genetic and microbiological parameters and therefore offers you efficient routine diagnostic testing.
Technique-brochure DNA•STRIP technology