GenoType EHEC

Your test system for determining shiga toxin genes, the intimin gene eae as well as the ipaH gene

Every year in Germany, there are about 1000 cases of illness due to an infection with enterohaemorrhagic E. coli (EHEC).
EHEC are characterized by very high infectivity. Generally 10-100 pathogens are enough to trigger an infection and thus also possible serious complications, such as, for example, haemolytic-uraemic syndrome. Improved laboratory diagnostics are therefore indispensable for the early detection of illnesses, in order to not only lessen the course of the disease, but also to avoid disease outbreaks, especially in shared facilities.
Microbiological detection of EHEC is problematic, since EHEC are not externally distinguished from apathogenic E. coli. Even the use of special nutrient media or serological determination detects only a part of the pathogen types described. The most important diagnostic criterion in the detection of an EHEC infection must therefore be the shiga toxin formed by the bacteria. This determination requires the use of molecular-biological methods.

With our GenoType EHEC test system, such a method is available to you for your reliable and rapid EHEC diagnosis. Starting from the primary culture, the shiga toxin genes that are important for reliable detection of EHEC are detected. Differentiating EHEC from shigella and EIEC is assured through the detection of the shigella-specific virulence factor ipaH.

reaction zones  GenoType EHEC

Your benefits with GenoType EHEC

  • Unambiguous result: a reliable differentiation of E. coli and Shigella ssp. as well as the detection of the diagnostically significant shiga toxin gene and the intimin gene eae provides you with unambiguous results and thus an efficient EHEC diagnosis.
  • Rapid result: detection is done using the primary culture within one workday and thus enables the rapid detection of EHEC.
  • Cost-effective: no cost-intensive instrumentation is necessary to perform the test; therefore, economic set-up is thus possible for smaller laboratories.

At a glance
Molecular genetic assay for identification of shiga toxin genes stx1 and stx2, the eae gene and the ipaH gene

Starting material:
primary culture, preferred bacteria freshly grown

DNA Isolation:
quick protocol (for further informaton please refer to the manual)

Order number: 
12 tests  No. 344
96 tests  No. 34496

Technology:
DNASTRIP technology

Downloads:
Flyer
Evaluation sheet
Overview equipment programs

Information request:
Enquiry

More Information:
More information about EHEC infections and their implications